Yes. Many, in fact. Let's take the auto industry.
It's the equivalent of buying a new car. You buy a new car and you get satellite radio and sweet shit on top of the purchase of the vehicle. Buy used, and you won't get cool features like that because you aren't directly supporting x car maker, you're supporting x car dealer.
This is the same idea. If you buy new, and support WB games and Rocksteady, you get Catwoman playable. After all, you're a paying support of Rocksteady. Catwoman is the satellite radio equivalent. Not required, but a bonus and it comes with the game for free if you buy new. If you buy used, you are supporting whoever you bought it from -- in most cases, Gamestop -- and in order to play as Catwoman you need to shell out some bucks on top of that used game. Makes sense.
This punishes used games sells and most definitely punishes used game sellers by taking back customers that are lost in the used game market.
You guys should be glad they're doing this as it's a total fuck off to Gamestop, who sucks. Why are you even complaining? Buy new, no problems. The game will likely be available for 40 bucks new on amazon in one month or two anyways, so stop bitching and shut the fuck up. The only people who are being nickel and dimed in this case are people who are buying used. If you buy new, this does not affect you in any way.
Batman: Arkham City is a fine way of handling dlc. Saints Row the Third and its multiple dlc's (cheats have to be bought through dlc now fyi) aren't.